Figure 5 | Scientific Reports

Figure 5

From: LLL12B, a small molecule STAT3 inhibitor, induces growth arrest, apoptosis, and enhances cisplatin-mediated cytotoxicity in medulloblastoma cells

Figure 5

(A) Knock down of STAT3 by STAT3-specific siRNA inhibits STAT3 phosphorylation, STAT3 protein level and cell viability in D283, D425, UW426, and UW288 human medulloblastoma cell lines compared to Control siRNA. (B) Inhibition of STAT3 phosphorylation and cell viability by LLL12B or STAT3 siRNA. Cells were seeded for overnight, and transiently transfected with 100 nM of STAT3 siRNA or control siRNA for 24 h. After that, cells were cultured for 48 h then were treated with LLL12B (0.5 μM) or DMSO for additional 48 h. Cell viability was measured using MTT assay. Experiments were performed in triplicate and data are presented as means ± SE. ***P < 0.001.

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