Figure 3
Ki67 changes after therapy in PDX. The reactions were carried out in an automated immunostaining platform (Autostainer Link AS48, Agilent). The slides were incubated with mouse monoclonal anti-KI-67 (MIB-1, Ready to use, Agilent, IR626). After the primary antibody, slides were incubated with the visualization systems (EnVision FLEX + Rabbit or Mouse linker, Dako) conjugated with horseradish peroxidase. Immunohistochemical reaction was developed using 3, 30-diaminobenzidine tetrahydrochloride (DAB) including in FLEX Kit and nuclei were counterstained with Carazzi’s hematoxylin. Finally, the slides were dehydrated, cleared and mounted with a permanent mounting medium for microscopic evaluation. ns, nonsignificant; *p < 0.05; **p < 0.005; ***p < 0.0005; Dc, docetaxel; Cb, carboplatin; V, vehicle; Dx, doxorubicin; Dc + Cb, docetaxel + carboplatin.
