Table 1 2D spots with significant (p ≤ 0.05) fold changes from the newly acquired N. perurans culture identified by LC–MS/MS from N. perurans. The complete list of proteins identified from the large collated database containing N. perurans microbiome, Amoebozoa and N. perurans proteins are in Supplementary Table S2. Several proteins that are shared between N. perurans and species found in the bacteria and Amoebozoa database are distinguished in the organism header of Supplementary Table S2. N. perurans GenBank IDs can be found in Supplementary Table S1.
Estimated MW (kDa) | Estimated pI | Fold change | p value ≤ 0.05 | Protein identification | No peptides matched | Coverage (%) | Biological function |
|---|---|---|---|---|---|---|---|
20 | 5.61 | 4.6 | 0.005 | ATP synthase subunit mitochondrial-like | 2 | 6 | Metabolism |
20 | 5.61 | 4.6 | 0.005 | Histone H2B | 2 | 18.3 | Protein synthesis |
20 | 5.61 | 4.6 | 0.005 | ADP-ribosylation factor 4 and 1 | 2 | 25.8 | Cellular signalling |
40 | 5.55 | 3 | 0.042 | Heat shock protein 8 | 3 | 7.1 | Stress response |
70 | 4.45 | 2.5 | 9.55E−05 | Peptidase C53 family protein | 4 | 9 | Immune evasion |
70 | 4.45 | 2.5 | 9.55E−05 | Carbohydrate ABC transporter substrate-binding protein | 3 | 2.5 | Metabolism |
16 | 6.12 | 4.4 | 1.10E−04 | Actin, cytoplasmic A3a isoform | 8 | 22.6 | Cytoskeleton |
21 | 5.95 | 2.1 | 0.003 | Cu–Zn Superoxide dismutase | 2 | 16.3 | Oxidative response |
21 | 5.95 | 2.1 | 0.003 | ADF-like domain-containing protein | 2 | 15.5 | Cytoskeleton |
21 | 5.95 | 2.1 | 0.003 | Lipoxygenase (lox) homology domain-containing protein 1 | 2 | 26.4 | Cellular signalling |
17 | 6.08 | 7.2 | 6.25E−04 | Profilin conserved site domain-containing protein | 6 | 64.3 | Cytoskeleton, immunomodulation |
17 | 6.08 | 7.2 | 6.25E−04 | Profilin allergen | 6 | 44.4 | Cytoskeleton, immunomodulation |
36 | 5.61 | 5.2 | 0.007 | Malate dehydrogenase | 4 | 13.8 | Metabolism |
45 | 5.36 | 1.9 | 0.033 | Fragmin A | 3 | 12.8 | Cytoskeleton |
45 | 5.36 | 1.9 | 0.033 | Elongation factor 1 | 2 | 17.4 | Cytoskeleton |
45 | 5.36 | 1.9 | 0.033 | Component of cytosolic 80S ribosome and 60S large subunit | 2 | 7.7 | Translation |
45 | 5.36 | 1.9 | 0.033 | Citrate synthase active | 2 | 5.6 | Metabolism |
142 | 4.5 | 3.3 | 0.018 | Aconitate hydratase | 2 | 2.4 | Oxidative stress |
