Table 1 Sequences and their respective ΔG values (RBS: ribosome binding site of pET22b vector).

From: Scale-up fermentation of Escherichia coli for the production of recombinant endoglucanase from Clostridium thermocellum

Sequence (RBS >  + 10 codon)

ΔG (kcal/mole)

Native

 − 4.0

AAGGAGATATACAT ATG GTA TCA GGC AAT TTG AAG GTT GAA TTC

RBS + 1 + 2 + 3 + 4 + 5 + 6 + 7 + 8 + 9 + 10

AAGGAGATATACAT ATG GTT TCA GGC AAT TTG AAG GTT GAA TTC

 − 2.8

AAGGAGATATACAT ATG GTG TCA GGC AAT TTG AAG GTT GAA TTC

 − 4.0

AAGGAGATATACAT ATG GTC TCA GGC AAT TTG AAG GTT GAA TTC

 − 5.4

AAGGAGATATACAT ATG GTA TCT GGC AAT TTG AAG GTT GAA TTC

 − 4.8

AAGGAGATATACAT ATG GTA TCG GGC AAT TTG AAG GTT GAA TTC

 − 4.0

AAGGAGATATACAT ATG GTA TCC GGC AAT TTG AAG GTT GAA TTC

 − 4.3

AAGGAGATATACAT ATG GTA TCA GGT AAT TTG AAG GTT GAA TTC

 − 2.8

AAGGAGATATACAT ATG GTA TCA GGA AAT TTG AAG GTT GAA TTC

 − 2.8

AAGGAGATATACAT ATG GTA TCA GGG AAT TTG AAG GTT GAA TTC

 − 4.8

AAGGAGATATACAT ATG GTA TCA GGC AAC TTG AAG GTT GAA TTC

 − 6.4

AAGGAGATATACAT ATG GTA TCA GGC AAT TTA AAG GTT GAA TTC

 − 4.0

AAGGAGATATACAT ATG GTA TCA GGC AAT CTG AAG GTT GAA TTC

 − 6.1

AAGGAGATATACAT ATG GTA TCA GGC AAT TTG AAA GTT GAA TTC

 − 3.8

AAGGAGATATACAT ATG GTA TCA GGC AAT TTG AAG GTA GAA TTC

 − 2.8

AAGGAGATATACAT ATG GTA TCA GGC AAT TTG AAG GTG GAA TTC

 − 2.8

AAGGAGATATACAT ATG GTA TCA GGC AAT TTG AAG GTC GAA TTC

 − 4.8

AAGGAGATATACAT ATG GTA TCA GGC AAT TTG AAG GTT GAG TTC

 − 4.0

AAGGAGATATACAT ATG GTA TCA GGC AAT TTG AAG GTT GAA TTT

 − 4.0

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