Table 2 Quadratic model predicted responses of the fermentation parameters by using central composite design.

From: Scale-up fermentation of Escherichia coli for the production of recombinant endoglucanase from Clostridium thermocellum

Run

Space type

Temperature (°C)

pH

Agitation (rpm)

Cell growth (g/l)

CMCase activity (U/ml)

1

Center

(0)37

(0)7

(0)200

6.5

1165

2

Center

(0)37

(0)7

(0)200

6.5

1165

3

Factorial

(− 1)28

(− 1)5.5

(+ 1)250

2.2

130

4

Factorial

(+ 1)46

(+ 1)8.5

(− 1)150

1.5

325

5

Center

(0)37

(0)7

(0)200

6.5

1165

6

Factorial

(− 1)28

(+ 1)8.5

(− 1)150

1.8

140

7

Factorial

(+ 1)46

(− 1)5.5

(− 1)150

2.5

280

8

Center

(0)37

(0)7

(0)200

6.5

1165

9

Factorial

(− 1)28

(− 1)5.5

(− 1)150

2.8

320

10

Factorial

(+ 1)46

(+ 1)8.5

(+ 1)250

2.5

330

11

Factorial

(− 1)28

(+ 1)8.5

(+ 1)250

3.5

270

12

Factorial

(+ 1)46

(− 1)5.5

(+ 1)250

2.5

155

13

Axial

(0)37

(+ α)9.52269

(0)200

3.5

250

14

Center

(0)37

(0)7

(0)200

6.5

1165

15

Axial

(0)37

(0)7

(− α)115.91

3

300

16

Center

(0)37

(0)7

(0)200

6.5

1165

17

Axial

(+ α)52.1361

(0)7

(0)200

0.65

80

18

Axial

(0)37

(0)7

(+ α)284.09

3.5

345

19

Axial

(0)37

(− α)4.47731

(0)200

2

110

20

Axial

(− α)21.8639

(0)7

(0)200

0.7

95

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