Figure 5
From: Degradation of insulin amyloid by antibiotic minocycline and formation of toxic intermediates
Identification of the binding site of minocycline to insulin amyloids. (A) Scheme of a model of insulin amyloid blocked by fluorescence probes at the minocycline binding site. (B) BCA assay of supernatant samples of (i)-amyloid and (r)-amyloid blocked by ThT (left) and LCOs (right) to confirm the identification of the binding site of minocycline in insulin amyloid: (i)-amyloid (gray) and (r)-amyloid (white). pFTAA and BTD 21 were used for (i)-amyloid and (r)-amyloid, respectively. Protein concentration was normalized for 50 µM (all insulin amyloid concentration).
