Figure 3

Expression profiles of the Gnrh system in fed and fasted conditions of wild-type zebrafish. Relative mRNA levels of gnrh2 (a) and gnrh3 (b) from brain tissues. Protein content of Gnrh2 in pituitaries from fed and 14-day fasted male and female wild-type zebrafish (c). Relative mRNA levels of gnrhr1 (d), gnrhr3 (e), and gnrhr4 (f) in pituitaries from fed and fasted male and female wild-type zebrafish. All data expressed as means ± S.E.M, n = 8 pituitaries or brains per group, *P < 0.05.