Figure 1
From: Retinitis pigmentosa is associated with shifts in the gut microbiome
Retinal changes in RP mice. (a) Scotopic ERG responses to 1 log cd s/m2 flashes from a normal C57BL/6J (left) and dystrophic rd10 (right) mouse. The amplitudes of both the a- and b-waves are represented. (b, c) Luminance-response curves for the a- (b) and b-(c) waves of C57BL/6J (circles) and rd10 mice (squares). (d) Configuration of the optomotor system (left, image created using BioRender; https://biorender.com/) and visual acuity thresholds for C57BL/6J and rd10 mice (right). (e) Mean outer nuclear layer thickness in C57BL/6J (circles) and rd10 (squares) mice, quantified in both the temporal and the nasal side of the retina. (f, g) Retinal sections showing the outer retina of a C57BL/6J (f) and rd10 (g) mouse immunolabeled against cone arrestin (cone cells, in green) and rhodopsin (Rho, rod cells, in red). Nuclei were stained with TO-PRO 3 (in blue). (h–k) Retinal sections from a C57BL/6J (h, j) and rd10 (i, k) mouse, immunolabeled against Iba1 (microglia, in green) or GFAP (activated macroglia, in red). The cell nuclei were stained with TO-PRO 3 (in blue). ANOVA, Bonferroni’s test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ON: optic nerve, OS: outer segments, ONL: outer nuclear layer, OPL: outer plexiform layer, INL: inner nuclear layer, IPL: inner plexiform layer, GCL: ganglion cell layer. Scale bars: (f, g), 20 µm; (h–k), 50 µm.
