Figure 2
From: The nickel-chelator dimethylglyoxime inhibits human amyloid beta peptide in vitro aggregation
Time-dependent Aβ40 peptide aggregation in absence or presence of Ni(II) and DMG at various pHs. Aβ40 peptide (25 µM) and thioflavin (40 µM) were mixed in absence of metal or DMG (circles), in presence 25 µM Ni(II) (squares), or in presence of 10 µM Zn(II) (triangles). The final pH in the reaction was 6.5 (white symbols), 7.5 (grey symbols), or 8.5 (black symbols). For instance, black triangles represent RFUs measured in presence of Zn(II) at pH 8.5. ThT-based fluorescence was measured every 3 min for 120 min. A ThT-only background control (no Aβ40 peptide) was included in the assay (data not shown). Results shown for each time point represent the mean and standard deviation (error bars) of background-subtracted values for triplicate wells.
