Figure 2
From: Autofluorescence enhancement for label-free imaging of myelinated fibers in mammalian brains
Characterization of the protocol. (a) Fluorescence emission spectra of the different samples excited with TPFM at 800 nm. (b) Fluorescence lifetime distributions (mean ± std.err) of fiber (bottom) and tissue (top) during the MAGIC steps. (c) Box chart plot of fluorescence lifetime values. (d) FLIM representative images during the three steps of the MAGIC protocol. Lifetime color scale is set from 550 to 1350 ps. (e) Raman spectra (mean ± std.err) of fiber and tissue during the MAGIC steps. (f) Glycerol scores (mean ± std.err) calculated along with the three major bands (550, 850, and 1465 cm-1) of the glycerol Raman spectrum. Statistical significance: *< 0.05 **< 0.001 *** < 0.0005. Images were prepared using SPC Image 4.9.7 (www.becker-hickl.com), graphs were prepared using OriginPro 9.0 (www.originlab.com).
