Figure 4
From: Autofluorescence enhancement for label-free imaging of myelinated fibers in mammalian brains
Fibers' orientation in Control and Reeler Hippocampi. (a-d) Magnified insets of blue and yellow boxes in e and f, respectively. Green arrows point to neuronal cell bodies, magenta arrows to myelinated fibers. Scale bar = 50 μm. (e, f) MIP of the mesoscale reconstructions of a control and Reeler mouse hippocampus 60-µm-thick coronal section. Scale bar = 1 mm. (g, h) Images show the ODF maps obtained from analyzing the full 3D hippocampus reconstruction of the control and the Reeler mouse, sampling 16 vectors for each ODF. (i) In-plane and out-of-plane orientation of the single ODF obtained by analyzing all the vectors of the full mosaic reconstructions and each of its ROIs. Acronyms list = cc: Corpus Callosum; S1: Primary Somatosensory Cortex; DG: Dentate Gyrus; CA1, CA2, CA3: field CA1, CA2, CA3 of hippocampus; fr: Fasciculus Retroflexus; LPLR: Lateral Posterior Thalamic Nucleus. Images were prepared using Fiji (www.fiji.sc/Fiji) 20while ODF representations using MRtrix3 (https://mrtrix.readthedocs.io/en/latest/)26.
