Figure 2

Profile of the extracellular metabolites through experiments 1–8. The plots show substrate (glucose), product (ethanol and glycerol) and inhibitors (vanillin, furfural and acetic acid) concentrations measured during each fermentation. (a) YPD control, (b) addition of vanillin (0.5 g/L), (c) addition of furfural (1.25 g/L), (d) addition of vanillin (0.5 g/L) and furfural (1.25 g/L), (e) addition of acetic acid (3 g/L), (f) addition of vanillin (0.5 g/L) and acetic acid (3 g/L), (g) addition of furfural (1.25 g/L) and acetic acid (3 g/L), (h) addition of vanillin (0.5 g/L) furfural (1.25 g/L) and acetic acid (3 g/L). For fermentations 1–7, the plotted values are the average of two replicates and the error bars indicate the difference between the two replicates. Note that for fermentation 8, only one replicate was measured. This figure was produced using Python70.