Figure 1
From: Furry is required for cell movements during gastrulation and functionally interacts with NDR1
Fry loss-of-function and rescue experiments. 4-cell stage Xenopus embryos were injected into both dorsal blastomeres as indicated and fixed at stage 30 (St. 30). (a) Uninjected embryo. (b) Standard Control morpholino (St-MO) (15 ng) injected embryo. (c,d) fry-MO (15 ng) injected embryos exhibiting “Shortened axis” or “Shortened axis & Head-less” phenotypes, respectively. (e) Rescue experiment; fry-MO (15 ng) + FD + LZ mRNA (800 pg) co-injected embryo. (f) FD + LZ mRNA (800 pg) injected embryo. Representative embryos are shown. (g) Quantitation of the percentage of embryos showing the different phenotypes: “Not affected”, “Shortened axis” or “Shortened axis & Head-less”. Embryos were scored as “Shortened axis” when presented < 80% of the body length relative to the average length of control embryos. N: number of independent experiments, n: number of embryos. Data in graph is presented as mean. Statistical significance was evaluated using Chi-square test (****,††††p < 0.0001). * represents the comparison to the uninjected group and † represents the comparison to the fry-MO injected group.
