Figure 3
From: Furry is required for cell movements during gastrulation and functionally interacts with NDR1
Fry is required for normal blastopore closure. (a–h) Uninjected or fry-MO (15 ng) injected embryos were fixed at the indicated gastrulation stage for blastopore closure measurements. Representative embryos for each stage are shown. Gastrula stage embryos are oriented dorsal to the top and neurula stage embryos are oriented anterior to the top. (i) Left: Quantification of the blastopore closure measurements on fixed uninjected embryos, fry-MO (15 ng) injected embryos and fry-MO (15 ng) + FD + LZ mRNA (800 pg) co-injected embryos at the indicated gastrulation stage. Right: Image showing the blastopore area (blue) and the area of the vegetal hemisphere of the embryo (total area, yellow). The ratio of these two measurements is plotted. 0 = blastopore closed. N: number of independent experiments, n: number of embryos. The stage of injected embryos was established based on the stage of uninjected control embryos. Means and standard deviation are indicated. Each point represents a single fixed embryo. Statistical significance was evaluated using Kruskal–Wallis test and Dunn's multiple comparisons test (****,††††p < 0.0001). * represents the comparison to the uninjected group and † represents the comparison to the fry-MO injected group.
