Figure 6
From: Furry is required for cell movements during gastrulation and functionally interacts with NDR1
Loss of Fry affects morphological polarity and orientation of dorsal mesoderm cells. (a,b) Dorsal marginal zone explants were prepared from Xenopus embryos at early gastrula stage (St. 10.5) and the vegetal alignment zone (VgAZ)5, which corresponds to the axial mesoderm, was imaged at late gastrula stage (St. 13) to assess dorsal mesoderm cell morphology. (a) mem-mScarlet mRNA injected embryo (N = 2, n = 7). (b) fry-MO (15 ng) + mem-mScarlet mRNA co-injected embryo (N = 2, n = 7). N: number of independent experiments, n: number of explants. Scale bar: 100 μm. Representative explants are shown. (c,d) Cell orientation was quantified as the angle of the cell’s major axis with respect to the mediolateral axis (ML). The circles in the rose diagram refer to the percentage of cells that exhibited polarity angles for each bin. Orientation angles were binned from 0° to 90° in bins of 11.25°. A: anterior, P: posterior, ML: mediolateral. (c) mem-mScarlet mRNA injected embryo (total number of cells analyzed = 147). (d) fry-MO + mem-mScarlet mRNA co-injected embryo (total number of cells analyzed = 151). Statistically significant differences were found between groups, Chi-square test (****p < 0.0001). (e) Polarity index measurements of dorsal mesoderm cells calculated as the ratio between cell major axis and minor axis. (f) Cellular area measurements of dorsal mesoderm cells. (e,f) mem-mScarlet mRNA injected embryos (total number of cells analyzed = 660); fry-MO + mem-mScarlet mRNA co-injected embryos (total number of cells analyzed = 632). Each point represents a single cell. Statistical significance was evaluated using two-tailed Mann Whitney U-test. **** p < 0.0001 indicates statistically significant differences between groups. Means and standard deviation are indicated.
