Table 1 16S rRNA targeted PCR primer sequences used in this study and their expected amplicon sizes.

From: Colonisation of the colonic mucus gel layer with butyrogenic and hydrogenotropic bacteria in health and ulcerative colitis

Bacterial target

Forward Primer (5′–3′)

Reverse Primer (5′–3′)

Product Size (bp)

Concentration (nM)

References

D. propionicus

CGC GTA GAT AAC CTG TCY TCA TG*

GTA GKA CGT GTG TAG CCC TGG TC

1120

250

63

D. curvatus

GAT AAT CTG CCT TCA AGC CTG G

CYY YYY GCR RAG TCG STG CCC T*

1150

250

63

B. wadsworthia

CGT GTG AAT AAT GCG AGG G

TCT CCG GTA CTC AAG CGT G

207

200

64

R. hominis

TAC TGC ATT GGA AAC TGT CG

CGG CAC CGA AGA GCA AT

230

100

65

  1. *Denotes the use of a degenerate base pair. Y = C/T, R = A/G, S = C/G, W = A/T, K = G/T.
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