Figure 4
X-irradiated field area distribution of cells with DNA damage in the cell population. The X-irradiated field sizes of 0.81 mm2 and 1.89 mm2 were divided into 0.3 mm × 0.3 mm, and the X-irradiated field area distribution of cell with DNA damages in cell population was analyzed at 1 h (A), 4 h (B), 24 h (C), 48 h (D), post-irradiation. After that, we investigated the number of 53BP1 foci as DSB per cell in cells located in area having not contact (black bar) or contact (white bar) with non-irradiated area of a cell population. Since the areas with X-irradiated field seizes of 0.02 mm2 and 0.09 mm2 are totally in contact with non-irradiated area, there is no data for the area in not contact with non-irradiated area. The data were pooled from three or four independent experiments and the results were represented as mean ± standard error. These figures were created with KaleidaGraph ver.4.5.2.
