Figure 1
From: In vitro skin culture media influence the viability and inflammatory response of primary macrophages
Influence of culture medium composition on skin cell viability and proliferation and in vitro skin maturation. (A) Schematic of the experiments performed. (B) Proliferation and (C) viability of primary fibroblasts, keratinocytes and macrophages cultured in RPMI control (“RPMI ctrl”), skin differentiation 1 (“Skin diff. 1”) or skin differentiation 2 (“Skin diff. 2”) media. Values are normalized to fibroblasts, keratinocytes or macrophages cultured for 1 day in fibroblast (“HDF med”), keratinocyte (“HEK med”) or RPMI control medium, respectively. N = 3, triplicate measurements for fibroblasts and keratinocytes; N = 5, triplicate measurements for macrophages. Error bars represent standard deviation (two-way ANOVA, Tukey's multiple comparisons test). Statistical significance indicated with *p < 0.05, ***p < 0.001. (D) Representative histological images of skin models cultured in the three media after 7 days of air-lift culture. N = 3, triplicate measurements. Scale bars: 100 µm.
