Figure 4
From: In vitro skin culture media influence the viability and inflammatory response of primary macrophages
Evaluation of macrophage medium effects on epidermal structure of skin models. In vitro skin equivalents were generated by culture in skin differentiation medium 1 (“Skin diff. 1”) or skin differentiation medium 2 (“Skin diff. 2”) for 14 days at air-lift culture (upper row), and then further cultured for 7 days in RPMI control medium (“RPMI ctrl”) (lower row). Immunohistochemistry against loricrin was performed and counterstaining with hematoxylin followed. N = 2, triplicate measurements. Scale bars: 100 µm.
