Table 1 Sequence of the primers used for quantification of macrophage gene expression.
From: In vitro skin culture media influence the viability and inflammatory response of primary macrophages
Gene | Primer name | Sequence 5′ → 3′ |
|---|---|---|
Glyceraldehyde-3-phosphate dehydrogenase, GAPDH | GAPDH-forward | AGTCAGCCGCATCTTCTTTT |
GAPDH-reverse | CCAATACGACCAAATCCGTTG | |
C–C chemokine receptor type 7, CCR7 (CD197) | CD197-forward | GTGGTTTTACCGCCCAGAGA |
CD197-reverse | CACTGTGGTGTTGTCTCCGA | |
C-X-C motif chemokine 10, CXCL10 | CXCL10-forward | CAGTCTCAGCACCATGAATCAA |
CXCL10-reverse | CAGTTCTAGAGAGAGGTACTCCTTG | |
C–C motif chemokine 22, CCL22 | CCL22-forward | GCGTGGTGTTGCTAACCTTC |
CCL22-reverse | CCACGGTCATCAGAGTAGGC | |
Mannose receptor C type 1, MRC1 (CD206) | CD206-forward | GCTACCCCTGCTCCTGGTTT |
CD206-reverse | CGCAGCGCTTGTGATCTTCA |
