Figure 1
From: Specific contribution of neurons from the Dbx1 lineage to the piriform cortex
Dbx1 is upregulated in Lhx2 conditional knockout cortices. (a) According to qPCR, the expression levels of Lhx2 and Pax6 are significantly downregulated in the cKO (Lhx2f/f; Emx1Cre) cortex when compared with WT (Lhx2fl/+) at E13.5, while the levels of Dbx1 and Rln are significantly upregulated in cKO mice (n = 3–5; Lhx2, P < 0.001; Pax6, P = 0.0054; Dbx1, P = 0.0085; Rln, P = 0.0412). (b) LacZ staining on coronal sections of Dbx1LacZ cortices of WT (top) and cKO (bottom) embryos at E13.5. From anterior (A) to posterior (P), LacZ+ cells (blue) are specifically located in the pallial–subpallial boundary (PSB, arrowheads) in WT. An increased number of LacZ+ cells (indicated by unfilled arrowheads) was observed in the neocortex (NC) of the cKO. Scale bar, 200 µm. GE, ganglionic eminence; Th, thalamus.
