Figure 2
From: Specific contribution of neurons from the Dbx1 lineage to the piriform cortex
The contribution of cells from the Dbx1 lineage in piriform cortex. (a) Coronal sections at the level of the APC from Dbx1Cre:Ai14:Gad67GFP cortex at P30. Cells of the Dbx1 lineage were labeled by tdTomato, and inhibitory interneurons were labeled by GFP. GFP-positive and tdTomato-positive cells are indicated by green and white arrowheads respectively, while double-labeled cells are indicated by yellow arrowheads. (b) Quantification of the percentage of interneurons in the Dbx1 lineage (number of GFP+tdTomato+ cells/number of tdTomato+ cells). About 4% of the Dbx1 lineage cells are inhibitory interneurons, across the PC (n = 3). (c, d) Coronal sections of Dbx1Cre:Ai3 cortex at P7 at the level of the anterior piriform cortex (APC) and posterior PC (PPC), as indicated on the left. Cells of the Dbx1 lineage were labeled by YFP (green); nuclei were stained with DAPI (blue). The APC and PPC are further divided into dorsal and ventral subregions. Arrowheads mark the dorsal and ventral ends of the PC, and arrow marks the border between dorsal and ventral PC. (e) Density of cells of the Dbx1 lineage (number of YFP + cells/number of total cells) measured in a radial column in PC subregions. Dbx1 cell denisty is significantly higher in ventral PC regions (n = 3). LOT, lateral olfactory tract; OB, olfactory bulb; NC, neocortex; Hp, hippocampus. Scale bars, 100 µm (a) and 500 µm (c).
