Figure 3
From: Specific contribution of neurons from the Dbx1 lineage to the piriform cortex
Neurons of the Dbx1 lineage show spatial and laminar distribution preferences in PC. (a, b) Coronal sections of Dbx1Cre:Ai3 cortex at P7 at eight levels from anterior (A) to posterior (P). Arrowheads mark the dorsal and ventral ends of PC and arrow marks the border between dorsal and ventral PC. (c) Quantification of the number of YFP-expressing cells in dorsal and ventral PC at each level. Significantly higher numbers of YFP+ cells were found in the ventral PC compared to dorsal PC (level 1, p = 0.00036; level 2, p = 1.0085E-05; level 3, p = 0.00165; level 4, p = 2.442E-05; level 5, p = 0.00042; level 6, p = 0.00101; level 7, p = 0.00059; level 8, p = 0.2561; n = 3). (d, e) Quantification of laminar distribution of YFP+ cells. Most YFP+ cells were detected in layer 2 (L2), across the PC. (f) Immunostaining for Reelin (red) on coronal section of Dbx1Cre:Ai3 cortex (at level 4 as in c). Reelin staining is enriched in L2a. (g, h) Within L2, the percentages of YFP+ cells in L2a and L2b are shown across the PC (n = 3). Scale bar, 100 µm.
