Figure 6
From: Specific contribution of neurons from the Dbx1 lineage to the piriform cortex
Neurons of the Dbx1 lineage in the ventral APC preferentially project to the orbitofrontal cortex. (a) Green fluorescence (Alexa Fluor 488)-conjugated CTB was injected into the lateral orbitofrontal cortex (LO) in Dbx1Cre:Ai14 mice at P30. An injection site is shown in the coronal section (below). (b) CTB-labeled neurons (green) were detected throughout the APC (sections 1–4 from anterior to posterior). Neurons of the Dbx1 lineage were labeled by tdTomato (Tom + , red). (c) Significantly more CTB-labeled neurons were detected in the ventral APC than in the dorsal APC. (d) LO-projecting neurons (green) were enriched in L2 of the ventral APC. Green-only neurons are indicated by hollow arrowheads and yellow neurons (positive for both green and red) are indicated by solid arrowheads. (e) Among the total population of neurons in vAPC L2 (labeled by DAPI), about 4% were LO projection neurons (labeled by CTB). Significantly higher percentage of LO-projecting neurons was found within the neuronal population of the Dbx1 lineage (more than 50%) (n = 3). Scale bar, 500 µm (a), 100 µm (b) and 50 µm (d).
