Figure 3
From: A novel proteomics approach to epigenetic profiling of circulating nucleosomes
Nu.Q Capture-MS allows the analysis of histone PTMs from circulating nucleosomes. Box plot showing the abundance of unmodifiable histone peptides H3 (a), H4 (b), H2A1 (c) detected by Nu.Q capture-MS in plasma sample from healthy donors (n = 9) or CRC patients (n = 9). p-values were determined by student’s t- Test (*p < 0.05; **p < 0.01). The box plot shows the median and the 25th and 75th percentiles; the whiskers indicate the 5th and 95th percentiles. CRC patients (#1 to #9) and healthy donors (#10 to #18) were indicated by different sample numbers. (d) Heat map showing the histone PTMs peptides (listed in Supplementary Table 2) differentially abundant between CRC patients (n = 9) and healthy donors (n = 9); p < 0.05. Data are shown as Log2 ratio of histone PTM levels (log 2 intensity) for a given sample based on average PTM levels across all samples. Log 2 intensity refers to heavy peptide normalized intensity. (e) Volcano plot representing the significance (expressed as −log10(p-value)) as a function of the magnitude of fold change between CRC patients (n = 9) vs healthy donors (n = 9) of the 54 histone proteoforms identified in plasma using the Nu.Q Capture-MS. 23 were significantly dysregulated including 2 downregulated (green) and 21 upregulated (red) (p < 0.05). Each plot with a significant fold change is labelled and recorded in Supplementary Table 3A.
