Figure 2
Wnt5a stimulation increases the number of spines with perforated PSD and synaptic contacts in hippocampal slices and synaptosomes. Representative EM images of perforated PSDs from the CA1 region of hippocampal slices (a) and multiple synapses (b), in response to 2 h of treatment with Wnt5a. Quantification of the time-course effect of Wnt5a shows an increased number of synaptic contacts per 100 μm2 area at 2 h (b) and a time-dependent increase in the percentage of dendritic spines presenting segmented PSD (a). Scale bar: 250 nm. N = 3 independent animals; Control (61); 30 (51); 60(45); 120 (48) photos (*p 0.05, ***p < 0.0001). (c) Synaptosome fraction isolated from mouse brain and treated for 1 h with Wnt5a. Representative EM images showing the number of PSDs and the number of multiple synaptic contacts after treatment with control or Wnt5a conditioned medium and the graph bar showing the quantifications. N = 3 independent animals Control (50); Wnt5a (59) photos (***p < 0.0001). Scale bars: 1 μm and 250 nm, respectively.
