Figure 1

(A) Relative expression of rhg1 genes AAT (Glyma.18G022400) and SNAP (Glyma.18G022500), and Rhg4 gene SHMT (Glyma.08G108900). No clustering, scaling, or imputed values. Sequential color scheme shows FPKM values. (B) Probabilistic principal component analysis (PCA) plot of expression of genes SNAP (Glyma.18G022500) and SHMT (Glyma.08G108900). Individual points represent FPKM of treatment groups P–C (Peking control), P–T (Peking SCN treated), R–C (NRS100 control), R–T (NRS100 SCN treated), W–C (Williams 82 control), W–T (Williams 82 SCN treated), S–C (S-soja control), S–T (S-soja SCN treated). (C) Significantly up and down regulated genes by genotype. DEGs considered significant if q value < 0.01, log₂foldchange no less than ± 0.6. (D) Heatmap of all DEGs by log₂FC (fold change) of induced response to SCN treatment of all genotypes. Cutoff Log₂FC no less than ± 2 for NRS100, including 2076 genes. Green bar indicates NRS100 specific up regulated genes in response to SCN stress. Red bar indicates NRS100 specific down regulated genes in response to SCN stress. Blue bar indicates Peking specific up regulated genes in response to SCN stress. Orange bar indicates Peking specific down regulated genes in response to SCN stress.