Figure 3
From: Farm use of calcium hydroxide as an effective barrier against pathogens
The effect of water on disinfection. (a) E. coli abundance (log10 colony-forming units, CFU) after contact without (control) or with disinfectant (CaCO3, CA(OH)2, or SLBGs) with no water added (dry condition) for 3 h. The dashed line indicates the detection limit estimated from the dilution ratio. (b) E. coli abundance after contact with disinfectant (CaCO3, Ca(OH)2, or SLBGs) with water added (wet condition) for up to 3 h. (c) E. coli abundance after contact without (control) with low pH Ca(OH)2 (in this Ca(OH)2, pH was reduced to about 9.28 in the door test for 63 days in Fig. 2d) with water added (wet condition) for up to 3 h. (d) Live/dead assay with disinfectant (control, CaCO3, Ca(OH)2, or SLBGs) in dry and wet conditions after 3 h. Images were captured using a fluorescence microscope equipped with a 20 × objective. Bar, 100 μm. Data in a, b, and c represent the mean and SD (error bars) from three separate experiments. Alive and Dead in a, b, and c indicate the range of CFU in which the number of colonies was observed and the range of CFU below the detection limit (2 log10 CFU/mL) in which no colonies were observed, respectively.
