Figure 3
Intravascular administration of dN4 and dC4 reduced bacteria numbers in lung tissue and reduced mortality associated with A. baumannii-induced pneumonia. (A) Mice were intratracheally infected with A. baumannii ATCC 17,978 at a dose of 5 × 108 CFU. dC4 or dN4 (2 mg/kg) was intravascularly administrated 4 h after bacterial infection. Infected mice were sacrificed 1 d after infection, and lung tissue was isolated. Bacteria that colonized lung tissue were quantified by plating on LB agar plates. Each point represents one mouse. Data represent means ± standard error of the means and were statistically analyzed using Mann–Whitney U-test versus the no treatment group. (B) Mice were intratracheally infected with A. baumannii ATCC 17,978 at a dose of 5 × 108 CFU. dC4 or dN4 (2 mg/kg) was intravascularly administrated 4 h after bacterial infection. Mouse survival was detected daily (n = 5/group). (C) Mice were intratracheally infected with A. baumannii ATCC 17,978 at a dose of 5 × 108 CFU. dC4 or dN4 (2 mg/kg) was intravascularly administered once a day for 3 d. Mouse survival was detected daily (n = 10/group).
