Figure 10

HYAL1 activity in NMR, GP, and mouse lymph nodes (a,b) and serum (c,d). HYAL1 activity was measured using native zymography in mouse, NMR, and GP (a) lymph nodes, and (c) serum (cropped gels). Relative HYAL1 activity was assessed in (b) lymph nodes (n = 3), and (d) serum (n = 3). Means ± SEM are shown. Differences are significant (***P < 0.001; **P < 0.01; *P < 0.05) in mouse vs NMR lymph nodes (Student’s t-test; no detectable activity was found in GP lymph nodes), and between the three species in serum (ANOVA and Bonferroni test). Full-length blots/gels are presented in Supplementary Fig. 2A,B.