Table 3 Primer sequences used for gene expression analysis by RT-qPCR assay.
Gene | Primer sequence (5′–3′) | Accession no |
|---|---|---|
Digestive enzymes | ||
AMY2A | F-CGGAGTGGATGTTAACGACTGG R-ATGTTCGCAGACCCAGTCATTG | NM_001001473.2 |
PNLIP | F-GCATCTGGGAAG↓GAACTAGGG R- TGAACCACAAGCATAGCCCA | NM_001277382.1 |
CCK | F-AGGTTCCACTGGGAGGTTCT R-CGCCTGCTGTTCTTTAGGAG | XM_015281332.1 |
Tight junction protein and gut barrier function | ||
Occludin | F-ACGGCAAAGCCAACATCTAC R- ATCCGCCACGTTCTTCAC | XM_031604121.1 |
ZO-1 | F-GCCAACTGATGCTGAACCAA R- GGGAGAGACAGGACAGGACT | XM_015278975 |
CLDN 1 | F-GGTGAAGAAGATGCGGATGG R- TCTGGTGTTAACGGGTGTGA | NM_001013611 |
MUC-2 | F-AAACAACGGCCATGTTTCAT R- GTGTGACACTGGTGTGCTGA | NM_001318434 |
JAM-2 | F-AGACAGGAACAGGCAGTGCT R- TCCAATCCCATTTGAGGCTA | XM_031556661.1 |
FABP2 | F-AGGCTCTTGGAACCTGGAAG R- CTTGGCTTCAACTCCTTCGT | NM_001007923 |
Immune related genes | ||
IL-2 | F: GCTTATGGAGCATCTCTATCATCA R: GGTGCACTCCTGGGTCTC | XM_015276098.2 |
IL-6 | F: AGGACGAGATGTGCAAGAAGTTC R: TTGGGCAGGTTGAGGTTGTT | NM_204628.1 |
IL-10 | F: GCTGAGGGTGAAGTTTGAGG R: AGACTGGCAGCCAAAGGTC | XM_025143715.1 |
IgA | F: ACCACGGCTCTGACTGTACC R: CGATGGTCTCCTTCACATCA | S40610.1 |
House keeping | ||
GAPDH | F: CAACCCCCAATGTCTCTGTT R: TCAGCAGCAGCCTTCACTAC | NM205518 |
TBP | F: GTCCACGGTGAATCTTGGTT R: GCGCAGTAGTACGTGGTTCTC | Acc:8484 |
Salmonella Typhimurium | ||
invA | F: GTGAAATTATCGCCACGTTCGGGCAA R: TCATCGCACCGTCAAAGGAACC | KF026356 |
