Figure 1

(a) The 3D reconstructed overview of the proximal segment of GFP transgenic murine vas deferens. (b) Lumens of (a). (c,d) Sagittal sections including the lumen of (a). (e): 3D reconstructed overview of the middle segment of GFP transgenic murine vas deferens. (f) Lumens of (e). (g,h) Sagittal sections including the lumen of (e). (i): 3D reconstructed overview of the distal segment of GFP transgenic murine vas deferens. (j): Side view of the mucosa area of (i). Front view of the mucosa area of (i) (inset at the upper right corner of (j)). (k,l): Sagittal sections including the lumen of (i). The same images following deconvolution and 3D reconstruction using the Avizo software (version 9.1.1) available at https://www.fei.com/software/avizo3d/ (a,b,e,f,i,j). LF longitudinal fold, TF transverse fold, M mucosa, L lumen, SM smooth muscle. Scale bars: 100 µm (c,d,g,h,k,l).