Figure 3
(a–f) Representative images of the double immunolabeling for PDGFRα (green) and CD34 (red) in the lamina propria (a–c) and in the smooth muscle layer (d–f). High magnification in the area beneath the epithelium (inset at the upper right corner of (c)). PDGFRα-immunoreactivity (IR) observed beneath the epithelium was not co-labeled with CD34-IR (c). PDGFRα-IR observed within the deeper portions of the lamina propria, in the smooth muscle and serosal layers were co-labeled with CD34 (c,f). (g–l) Representative images of the double immunolabeling for PDGFRα (green) and vimentin (red) in the lamina propria (g–i), in the smooth muscle layer and serosal layer (j–l). PDGFRα-IR was partially co-labeled with vimentin-IR in all layers (c,f). High magnification in the serosal layer (inset at the upper right corner of (l)). Vimentin-single IR cells were observed in the outermost of serosal layer (f). Nuclei were counterstained in blue with 4′,6-diamidino-2-phenylindole (c,f,i,l). Scale bars: 20 µm (a–l), 5 µm (inset at the upper right corner of (c,l)).
