Figure 1 | Scientific Reports

Figure 1

From: An EAV-HP insertion in the promoter region of SLCO1B3 has pleiotropic effects on chicken liver metabolism based on the transcriptome and proteome analysis

Figure 1

Dual luciferase assays to verify chicken IR-1 like element. (A) Schematic diagram of promoter fragments of SLCO1B3 with different lengths constructed into pGL3.Basic vector. P1: − 202 ~  + 7; P2: − 533 ~  + 7; P3: − 709 ~  + 7; P4: − 917 ~  + 7; P5: − 1431 ~  + 7; P6: − 1831 ~  + 7. (B) Relative luciferase activity of serial constructed vectors with insertions of different length 5ʹ flanking regions of SLCO1B3 after transfection into LMH cells. (C) Expression levels of SLCO1B3 of LMH cells when cultured with different CDCA concentration in the medium. (D) Relative luciferase activity of serial constructed vectors with insertions of different length of 5ʹ flanking regions of SLCO1B3 after transfection into LMH cells with 50 μM CDCA in the complete medium. (E) The sequence of human SLCO1B3 IR-1 element, the predicted chicken IR-1 like element and its mutation type in this assay. Chicken predicted IR-1 element is not similar with the human IR-1 element and it’s located in the 5ʹ flanking region of SLCO1B3(-202 ~  + 7). (F) Relative luciferase activity of the − 202 ~  + 7 fragment, its predicted IR-1 like mutant constructed vectors (− 202 ~  + 7-Mut) and an IR-1 plus EAV-HP insertion fragment vector (− 614_IM+  ~  + 8) after transfection into LMH cells with 50 μM CDCA or DMSO in the compete medium. Different letters indicate significant differences (P ≤ 0.05).

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