Figure 1

Workflow of the generation of carbohydrate-binding module-assisted lateral flow assays: The carbohydrate-binding module 3a of Clostridium thermocellum (CBM) was fused to different single chain variable Fragments (scFv) or to full-length antibodies (IgG). The resulting CBM-fusion proteins, as well as the non-fused CBM, scFv and full-length antibody were examined regarding their binding properties towards cellulose paper. Cellulose-based lateral flow assays were performed and compared using the CBM-fused antibodies as well as the solitary antibodies.