Figure 2 | Scientific Reports

Figure 2

From: Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays

Figure 2

Cellulose and nitrocellulose binding studies using CBM protein, determining the influence of different buffer additives. 125I radiolabeled CBM protein was applied to (A) cellulose-paper stripes or (B) nitrocellulose membrane. After drying, application of buffer solution containing different additives, protein localization and quantitative analysis of bound and washed-off protein was analyzed using phosphor imaging (Figure S3). (C) Quantitative analysis of protein-binding towards cellulose and nitrocellulose was performed according to Figure S4. Standard derivation was calculated using data derived from experimental triplicates.

Back to article page