Figure 5

Cellulose-based lateral flow assays for the detection of SARS-CoV-2 specific antibodies in serum samples. (A) Experimental setup for the Covid19 antibody LFA using (B) CBM-anti-Fc scFv, (C) anti-hIgG-CBM, (D) anti-Fc scFv or (E) anti-hIgG functioning as detection antibody, printed on the test line area. 150 µl of sample was applied to the sample pad, containing 130 µl of TBS buffer,20 µl of human serum and different amounts of anti-SARS-CoV-2 spike antibody. Sample compositions are listed in Table S2.