Figure 5
From: Life-extended glycosylated IL-2 promotes Treg induction and suppression of autoimmunity
Effects of S2A on DSS induced colitis. (A) Mice (n = 12 per treatment) were given water supplemented with DSS, which induces colitis, for a week, during which mice were inoculated twice (day 2 and 6) with 30,000 units of S2A, Proleukin and a corresponding volume of vehicle. After this period, half of the mice were sacrificed (n = 6 per treatment), and half were left to enter a recovery period of a week before sacrifice (n = 6 per treatment). (B) Mice were weighed throughout the experiment, and weight loss as a percentage of original weight was plotted against time (black filled down pointing triangle indicates inoculations). Two-way ANOVA was calculated using SPSS, where the combined effect of treatment and time was found to be significant (P < 0.0005). Post-hoc test revealed S2A was significantly different from Proleukin (P < 0.05) and vehicle (P < 0.0005). (C) In addition to weight loss, clinical data (diarrhea and anal bleeding) was collected and factored with weight loss to compile the clinical score, which is plotted against time (black filled down pointing triangle indicates inoculations). Two-way ANOVA was carried in SPSS, and the combined effect of treatments and time was found to be significant (P < 0.0005). The post-hoc test showed a significant difference between S2A and vehicle but not with Proleukin. However, when considering only the recovery period, statistical significance arises between S2A and Proleukin (P < 0.005). (D) A representative photograph of colons removed from sacrificed mice, at the end of the DSS period (day 8) and at the end of the recovery period (day 15). (E) Colon length was measured the following sacrifice, (continuous line—mean, dashed line—median). (F) Representative images of hematoxylin and eosin (H&E) stained colon tissues. (G) Quantification of H&E staining. (H, I) PBMCs were isolated during sacrifice day. FACS analysis is arranged according to lineage and presented as a percentage of the population noted on the y-axis. Error bars represent 95% CI. *, P < 0.05; **, P < 0.005; ***, P < 0.0005.
