Figure 2

Alkaline phosphatase (ALP) cytochemical staining of CH-MSCs and PL-MSCs cultured in osteogenic differentiation medium with or without BMP-2. (A,C) ALP cytochemical staining at different time points shows an increase in ALP activity (deep blue) during osteogenesis. (B,D) ALP enzyme activity assay shows a configuration similar to the ALP staining assay. Data calculated from three independent experiments are presented as mean± SEM. p<0.05 compared to MSCs cultured in complete DMEM medium marked as “a”. p<0.05 compared with MSCs cultured in osteogenic differentiation medium (OstDiff medium) marked as “b”. Scale bar = 100 μm.