Figure 8

Expression of alkaline phosphatase in CH-MSCs (A) and PL-MSCs (B) after treatment with anti-miR-31, anti-miR-106a, and anti-miR-148a. The alkaline phosphatase enzyme activity assay showed higher ALP activity in MSCs treated with miRNA inhibitors (C,D) Statistically significant differences with p<0.05 compared to MSCs cultured in osteogenic differentiation medium (OstDiff medium) supplemented with miRNA negative control are marked as “a”. p<0.05 compared to MSCs cultured in OstDiff medium supplemented with anti-miR-31, anti-miR-106a or anti-miR-148a are marked as “b”, “c” and “d” respectively. Scale bar = 100 μm.