Figure 6
Differential abundance of ASVs and microbiome function by ART regimen. Differentially represented ASVs in the HIV-positive NNRTI and PI/r cohorts were identified via paired, Wilcoxon tests. Mean relative abundances were calculated for these ASVs for the NNRTI and PI/r cohorts. In (A), the log-ratio of those mean abundances are plotted on the x axis, with ASVs less abundant in the PI/r cohort having a ratio less than 0. ASVs are grouped at the family level, and the genus-level taxonomic assignment is given on the y axis. ASVs with genus-level taxonomic assignments were filtered against the “List of Prokaryotes according to their Aerotolerant or Obligate Anaerobic Metabolism” and were assigned a binary value for predicted butyrate production and obligate anaerobic growth. Relative abundances for ASVs predicted to have these traits are shown in (B). There was a trending difference relative abundances of butyrate producers and obligate anaerobes by HIV status (p = 0.06). (C) Depicts the log-linear relationships between the relative abundance of these traits and the corresponding alpha diversity (p < 5e−8).
