Figure 2

CNNM2 depletion affects Mg²⁺ and Ca²⁺ homeostasis. (A) Serum Mg²⁺ is significantly reduced in newborn CNNM2 deficient mice compared to wild type littermates. Data are presented as mean ± SEM (n = 4–15) (B) Serum Ca²⁺ in newborn Cnnm2^−/− mice is significantly increased compared to Cnnm2^+/+ mice (n = 3–17) (C) Cnnm2^+/+ or Cnnm2^+/− mice were treated with low (0.02%), normal (0.23%), or high (0.48%) (w/w) Mg²⁺ diets for two weeks, followed by housing in metabolic cages. (D,F) Cnnm2^+/− mice (black bars) have lower serum Mg²⁺ levels and higher compared to wild type mice (white bars), independent of the Mg²⁺ diet. (E,G) Cnnm2^+/− mice have lower serum Mg²⁺ levels and an overall increase in serum Ca²⁺ levels compared to wild type littermates, independent of the Mg²⁺ diet. Serum Na⁺ (H) and K⁺ (I) levels in Cnnm2^+/− mice are unchanged (n = 10–12 per group). Data are presented as mean ± SEM. Significance determined with two-tailed Student’s T-test or Two-way ANOVA followed by a Tukey post-hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.