Figure 2
Plasma FGF21 level in plasma (A), brain FGF21 level (B), and brain FGF21 signaling cascades including a ratio of phosphorylated-FGFR1/FGFR1 (C) and β-klotho/actin (D) of β-thalassemic mice fed with either normal diet or high-iron diet. Three replicates of cropped representative bands for brain FGF21 signaling proteins are shown (E). In addition, full-length blots are presented in Supplementary Fig. 1. The samples derive from the same experiment and that gels/blots were processed in parallel. Actin was used as a loading control and the density of detected protein was divided by the density of actin from the sample. FGF21 fibroblast growth factor 21, FGFR1 fibroblast growth factor receptor 1, HFe high-iron diet fed mice, HT heterozygous-β knockout, ND normal diet fed mice. *p < 0.05 vs. HT-mice fed with normal diet.
