Table 4 Average ratio of the dry matter content of bee pollen samples P1-purified by different methods, before and after enzymatic P2 purification (removal of the intine wall and the cellular debris).

From: Facile isolation and analysis of sporopollenin exine from bee pollen

Type of pollen

P1 purification protocol

P2 residue %

Mixed

Soxhlet extractions

38.5

Hot aqueous and IPA washes

57.1

10% SLS and IPA washes

60.4

Rape

Soxhlet extractions

28.9

Hot aqueous and IPA washes

41.8

10% SLS and IPA washes

49.5

Sunflower

Soxhlet extractions

38.4

Hot aqueous and IPA washes

55.7

10% SLS and IPA washes

57.3

  1. Results of four experiments were averaged. The results are corrected with the losses occurring during the purification procedure. Losses were estimated by applying a similar purification protocol without using enzymes.
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