Figure 5
From: Ceramide kinase regulates TNF-α-induced immune responses in human monocytic cells
CERK downregulation attenuates the IL-1β and MCP-1 secretion in TNF-α activated monocytes. THP-1 monocytes and primary human monocytes were transfected with scrambled-siRNA (negative control; NC) or CERK siRNA and incubated for 36 h. CERK-downregulated THP-1 cells and primary human monocytes were treated with TNF-α for 12 h. Secreted IL-1β and MCP-1 proteins were measured in culture media using ELISA. IL-1β and MCP-1 secreted by THP-1 cells (A,B), and primary human monocytes (C,D). All data are expressed as mean ± SEM (n ≥ 3). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 versus vehicle.
