Table 1 Characteristics of the 26 loci developed for R. dauricum and R. mucronulatum.

From: Impact of different numbers of microsatellite markers on population genetic results using SLAF-seq data for Rhododendron species

Locus

Primer sequences (5′-3′)

Ta (°C)

Motif

Size (bp)

R. dauricum (N = 18)

R. mucronulatum (N = 10)

R. aureum (N = 12)

Total (N = 40)

NA

Ar

Hs

NA

Ar

Hs

NA

Ar

Hs

NA

Ar

A

F: CAGAAGACAAGCCTCTAAAT

51

(AG)13

204–252

6

3.762

0.503

1

1

0

1

1

0

7

4.233

R: GAGCCCAAAGATAACCAGTG

              

B

F: AGCAGTTTTGGAGCCAG

52

(AC)11

200–212

4

3.228

0.458

5

4.397

0.728

3

2.515

0.255

7

3.855

R: CTCAACATTTGCGAACACA

              

C

F: AGCCCTAGTCAGCTTCGTGT

56

(TGG)6

240–273

4

3.525

0.557

3

2.682

0.361

4

3.86

0.664

9

5.39

R: CCCAGAATTACCAAACCCTC

              

D

F: ACCTTTCCAACTCCCTGCTT

52

(AG)11

350–378

5

4.582

0.791

2

1.995

0.306

9

7.798

0.909

15

7.673

R: GTCGTCGTGTTTTCTCATTC

              

E

F: CGTGCATCTCCTTCGTCTAC

55

(CA)10

152–182

8

5.699

0.788

4

3.682

0.667

6

5.14

0.759

13

7.568

R: CAATCCGCCTATCAATCTGT

              

G

F: TCTTCTTTGCTTCACAGT

45

(TC)19

212–260

9

7.195

0.892

8

6.762

0.861

8

6.948

0.873

19

9.339

R: TATTTTCAGCCTTTTCCCAG

              

H

F: CAATATACCAGCATGTCATC

48

(TTC)10

339–357

5

3.986

0.511

4

3.739

0.653

4

3.604

0.506

9

5.841

R: CCTACAAGAGTTGGGAAATC

              

K

F: CCTTGTTGCATCAGAATCGA

54

(AGAC)5

164–192

4

3.415

0.596

1

1

0

1

1

0

4

3.329

R: TGACTAGGAGTGCTTCCACC

              

O

F: CTTCTCCACCGTCTGGCTCG

54

(CT)8

323–335

5

3.586

0.472

2

1.778

0.111

3

2.963

0.582

6

4.014

R: AGTTCTGTTGTTAGGGCTCC

              

R

F: ACGTGATGAAAGCTGGTTAT

51

(TC)8

118–144

6

4.32

0.655

6

4.721

0.517

5

4.831

0.795

14

7.605

R: GCTCGGGTTGTCTGGTTC

              

X

F: GTGAAGAGGACGAGAAAGGA

45

(GA)9

232–252

6

5.186

0.822

7

7

0.929

6

5.097

0.767

11

7.188

R: TCATGCCATATGTGCAAACG

              

Y

F: ACCCTAACTTAACATCTTCG

45

(TC)10

250–278

2

1.98

0.324

3

2.982

0.594

3

2.869

0.473

8

5.635

R: ACAAATGACATCAGCACTCT

              

AD

F: ACCTGCGGTCTTCAGTGCTT

56

(CT)7

252–282

5

3.887

0.616

3

2.982

0.65

4

3.273

0.591

10

6.447

R: GCCTTACAATATCCGTGTCG

              

AE

F: AGAGGGAACCAACAATACGA

54

(AG)8

188–206

6

4.715

0.788

4

3.621

0.583

2

1.636

0.091

10

6.225

R: ACGACCACCTGATGTGCGAC

              

AF

F: GGTTTGGGTTTTGAGGAATG

54

(GA)11

189–229

4

2.952

0.381

1

1

0

8

6.509

0.777

12

5.003

R: CTTGAAGCTGAGCTGAGTTA

              

B1

F: GTTTTACAAGACCAGAGCGT

53

(AG)11

168–270

7

5.623

0.842

4

3.665

0.561

5

4.942

0.827

9

6.806

R: CATTTCCGTTTCCTTCAGTC

              

B8

F: TAAATGGCTAATCGTCCTAC

54

(CT)13

410–426

3

2.319

0.297

2

1.997

0.356

1

1

0

4

3.023

R: AGCAAGTACAGCTCCGATGG

              

B10

F: AGCCGACCCTTATGCATTAT

54

(CT)7

284–326

6

3.95

0.655

6

5.097

0.761

9

7.099

0.864

15

6.773

R: CAAGCCCCCATCCTTTATCA

              

B11

F: GGATCAAGAAGGTGGTCAAT

53

(GA)6

322–348

5

3.973

0.654

4

3.962

0.683

3

2.963

0.591

7

4.898

R: CCAGTCAGCAATCAATAAGC

              

B12

F: GCAAAATCATAACAAAAGCA

52

(CT)11

298–332

12

8.237

0.91

3

2.992

0.679

9

7.415

0.882

15

8.422

R: AGCAGGAAACCCTATAAACA

              

B13

F: GCGTTCAAAATCTCCAGAGC

56

(AG)7

318–340

7

5.317

0.814

7

6.608

0.839

3

2.921

0.594

12

6.599

R: AGAACCCACTTGGATGCTGT

              

B14

F: TTTAGAAAGGTCACTGACAC

47

(TG)7

270–306

6

5.218

0.781

6

5.524

0.806

5

4.236

0.695

13

7.182

R: CTTGAAGTAATCGGCTATGT

              

B16

F: AGTTGAGCAAGACAAGTGGA

54

(GA)8

266–290

8

5.167

0.773

2

2

0.489

8

6.973

0.886

13

7.341

R: GCCTTCATTATGAAGTGGGT

              

B17

F: GAGAAGGACGGGCATTTACC

56

(TC)8

218–238

3

2.509

0.304

4

3.679

0.6

4

3.564

0.514

8

5.247

R: GGGCAGTTTTCCACTCATAC

              

B19

F: ATCTGGAACAAACAGGACAT

52

(TTC)6

347–362

3

2.892

0.484

3

2.922

0.396

4

3.758

0.664

5

3.601

R: CCATTATCGCTCTGAGTGTC

              

B21

F: CGGTTCCATTTTCTGACTGG

56

(GA)7

148–202

11

7.935

0.917

6

5.32

0.811

12

9.745

0.968

19

8.904

R: GTTTCTTTGGTTTTGGCTCT

              
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