Figure 3 | Scientific Reports

Figure 3

From: Ultrasensitive nucleic acid detection based on phosphorothioated hairpin-assisted isothermal amplification

Figure 3

Sensitivity of the PHAmp reaction. (a) Time-dependent fluorescence intensities (via SYBR Green I staining) during the PHAmp reaction with target DNA at various concentrations. (b) Linear relationship between Tt and the logarithm of the target DNA concentration in the range from 1fM to 1 nM, where Tt is defined as the time at which the fluorescence signal reaches the threshold intensity (1000 a.u.) and Ctarget is the target DNA concentration. The error bars represent the standard deviations of three replicate measurements.

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