Figure 4

The miRNA array analysis of EVs collected from human serum. (a) A schematic diagram of the miRNA array analysis of HepG2-EVs collected from human serum and urine using four EV collection methods (UC, MB, PR, and the AGC device). (b) The western blotting of CD9 in serum (upper), and CD9 in the collected and flow fraction of serum treated by AGC device (lower). These blots were cropped from the same gel. The black dot line shows the delineation with dividing from different parts of the same gel. All information about full-length gels and blots was provided in Fig S9. (c) The capture rate (%) of EVs using AGC device calculated by CD9/CD63 ELISA kit. (d) A gel image of nucleotides [nt] of total RNA extracted from EVs collected from human serum using four EV collection methods (UC, MB, PR, and AGC device) measured by a 2100 Bioanalyzer. (e) The concentration of total RNA extracted from EVs collected from human serum using four EV collection methods (UC, MB, PR and the AGC device) measured by a 2100 Bioanalyzer. (f) The number of miRNAs extracted from EVs collected from human serum using four EV collection methods (UC, MB, PR, and the AGC device). (g–i) A venn diagram of the overlap of the number of detected miRNAs between UC (as the standard method) and the other three methods (MB [g], PR [h] and the AGC device [i]).