Figure 3
From: Delay in primordial germ cell migration in adamts9 knockout zebrafish
Strong expression of Adamts9 in preovulatory follicles, weak or no expression in immature ovarian follicles or ovulated oocytes in Adamts9 transgenic lines (Tg(adamts9:GFP)). The expression of Adamts9 was determined by EGFP expression driven by adamts9 promoters located in a 4.5 kb upstream sequence of adamts9 start codon. Representative confocal slice images of stage I (immature pre-vitellogenic), III (immature vitellogenic), IVb (mature & preovulatory) follicles and stage V ovulated oocytes were shown. Follicles or oocytes were confocal imaged under EGFP and transmit light (T-PMT) channels. Similar expression was confirmed in the follicles or oocytes of several F1 and F2 adult females from five independent transgenic lines. Scale bar: 10 μm (stage I); 100 μm (stages III, IVb, V).
