Figure 5
From: Buprenorphine alters microglia and astrocytes acutely following diffuse traumatic brain injury
Microglia demonstrate a more active morphology in the cortex, but not the thalamus, following Bup-SR-Lab treatment. Representative photomicrographs of (A,B) cortex and (B,C) thalamus from rats 1d post-cFPI and treated with (A,C) saline or (B,D) buprenorphine (Bup). The microglial calcium binding protein, Iba-1, is labeled in green. Bar graphs depicting microglial morphological characteristics indicative of activation; (E) number of process end points/cell, (F) average soma size, (G) average maximum process length and (H) the average complexity of the microglial process network/cell. Microglial morphologies were significantly different in the cortex compared to microglia found within the thalamus, regardless of treatment. Additionally, microglial somal size and process network complexity was altered in the cortex, but not the thalamus of Bup-treated rats, suggesting regional specificity in the effects of Bup. Figure was compiled using Adobe Photoshop CS., version 22.0 (2020), San Diego, CA. Treatment differences: *p < 0.05 compared to saline; regional differences: #p < 0.05 compared to cortex. n = 6 rats/group. Mean ± SEM. Scale = 50 μm.
